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Development of a validated analytical method for Aceclofenac and Paracetamol injection by HPLC method

Kaaviya J1, B. Babu*,1, Priyadharseni R1, Ramesh J1, Meyyanathan S.N1

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ABSTRACT:

 

A simple, accurate, rapid and precise and validated HPLC method has been developed for the simultaneous estimation of Aceclofenac and Paracetamol injection by using C18 (250mm x 4.6 6 mm id., 5µm) using 0.3M Ammonium acetate: Methanol: ACN in the ratio (27:33:40v/v) as the mobile phase at a flow rate 1ml/min and detected at 260nm. The methods were validated in the terms of linearity, accuracy, specificity, range and precision. The method was found to be precise (%RSD<2) and the method was found to be linear for aceclofenac and paracetamol (>0.99). % recovery for aceclofenac and paracetamol was found to be within the limit.

 

KEY WORDS:

 

Aceclofenac, Paracetamol, HPLC, Validation and Method development